Chemical modification of castor bean hemagglutinin with diethylpyrocarbonate.
نویسندگان
چکیده
منابع مشابه
Chemical modification studies on Ricinus communis (Castor Bean) agglutinin.
Ricinus communis agglutinin was subjected to various chemical treatments and the effect on its hemagglutinating and saccharide-binding properties was studied. Acetylation, succinylation and citraconylation led to a complete loss in the activity of the agglutinin, whereas reductive methylation had no effect on the activity, showing that charged amino groups were involved in the hemagglutinating ...
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In 2009 a National Security Science and Technology grant was awarded to the Human Protection and Performance Division for the investigation of several forensic aspects of the castor bean plant Ricinus communis. A major focus of this grant was to understand the chemical composition of the seeds, and to ascertain if these differences could be used for provenance classification. This technical rep...
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The amounts of the two lectins (ricin and Ricinus communis agglutinin) in tissues of castor bean seedlings were followed during germination and early growth. For measurement, lectins in extracts were separately eluted from Sepharose columns; an antibody to the agglutinin was also used to detect the lectins by immunodiffusion. The endosperm of the dry seed contains 3.5 mg total lectin (5.6% of t...
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The endosperm of castor bean seeds (Rkinus comunuis L.) contains two -SH-dependent amptidases, one hydrolyzing L-leucine-,Bnaphthylamide optimally at pH 7.0, and the other hydrolyzing L-proline,B-naphthylamide optimally at pH 7.5. After germination the endosprm contains in addition an -SH-dependent hemoglobin protease, a serinedependent carboxypeptidase, and at least two -SH-dependent enzymes h...
متن کاملPhosphatidylcholine synthesis in castor bean endosperm.
Three pathways for phosphatidylcholine synthesis were assayed in castor bean (Ricinus communis var. Hale) endosperm. Phosphatidylethanolamine: S-adenosylmethionine methyl transferase occurred predominantly in the endoplasmic reticulum fraction, but some activity appeared in the mitochondria. Phosphorylcholine glyceride transferase occurred exclusively in the endoplasmic reticulum. The phosphory...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1989
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.53.735